RNASeq Data preprocessing using Shell commands in linux

Description

• Description
• Who is this course for
• What we offer
• Pricing

Next generation sequencing (NGS) allows us to establish a better understanding of biological processes through providing a wide overview of the genome and gene expression. The raw data of any NGS experiment is provided in a file format named FASTq, handling such files is essential for any bioinformatician. To achieve this task, shell commands in a Linux system are commonly used.   

In this you will be familiarised with the linux system, shell commands, data file formats ,selection and retrieval of raw files from GEO, as well as preprocessing of FASTq files in terms of  quality control, alignment to a reference genome , and generation of gene expression matrices.  

Learning objectives:

• Linux for NGS data analysis
• Data Retrieval (NCBI SRA)
• Understanding Fastq files and other data types
• Quality control check
• Retrieval of proper reference genome (Human, Mouse)
• Alignment of reads using reference Genome.
• Generation of gene expression matrices.

B.Sc. / M.Sc./ PhD in any field related to Biology, Pharmacy, Agriculture, or Medicine. 

• Focused, Interactive, and personalised workshops offering perfect learning experience.
• Flexible in time and place as the workshops are offered virtually.
• Training is performed on our optimised servers which eliminates the need for powerful hardware.
• Up to 15 hours of free access to our servers to implement what you learned.
• Certification of completion.